Recommended Concentration. For research use only. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. normal mouse IgG3 Alexa Fluor 594 est recommandé en immunoglobuline de contrôle négatif à la place d'un anticorps primaire spécifique de même isotype (mouse IgG en IF, IHC et FCM Not for resale. ICC staining of Alexa Fluor® 594 anti-ATG5 antibody (clone 177.19) on Hela cells. Negative control for use in conjunction with R&D Systems antibodies. Some can be used in single molecule studies . By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. 0.25-1 µg/10 6 cells. The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C. Alexa Fluor® 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. Do not freeze. No cross-reactivity has been detected against non-immunoglobulin serum proteins. No. Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. View full statement regarding label licenses, View information about IgG on UniProt.org. Each lot of this antibody is quality control tested by immunocytochemistry. Product Usage Information . TFAX 594, SE. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation. Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity) Antibody - Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. Excitation/Emission: 590⁄617: Labeling Scale: 100 µg: Color: Red: Label or Dye: Alexa Fluor™ 594 Do not freeze. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150112) HeLa cells showing negative staining by ICC/IF using only secondary … The cells were then incubated with 2 µg/mL of the primary antibody overnight at 4°C. Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 594), pre-adsorbed. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies. Flow Cytometry. Sample. Recommended Concentration. Alexa Fluor 594 conjugate of WGA exhibits the bright, red fluorescence of the Alexa Fluor 594 dye (excitation/emission maxima ∽590/617nm). Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. THANKS. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. This product is for IN VITRO RESEARCH USE ONLY. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Sample. Control. Not for diagnostic use. Then the cells were intracellularly stained overnight at 4°C with (A) 1: 100 diluted (5 µg/ml) Alexa Fluor® 594 rat IgG2a (Cat. Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The secondary antibody (orange) was ab150112 Alexa Fluor® 594 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. Exportable as image or link. Superior Customer Support. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Vortex or pipet up and down until fully dissolved. A431 human epithelial carcinoma cell line. 422801) to visualize nuclei. View full … Santa Cruz ARID2 (E-3) Alexa Fluor 594. Sample. Alexa Fluor® 594 is available conjugated to: Alexa Fluor® 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. Application: ELISA, Flow … Cite. The streptavidin-Alexa Fluor® 594 solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 610/20 bandpass filter. Packing: 200 ug. Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150128) HeLa cells showing negative staining by ICC/IF using only secondary … Alexa Fluor® 594 anti-Alix Antibody - Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 594 used at the same concentration and conditions as the primary antibody. .. Colominic acid sodium salt was purchased from Seikagaku Corp. (Tokyo, Japan). The average molecular weight of Streptavidin-Alexa Fluor® 594 is 60 kD and Streptavidin alone is 52 kD. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. Nuclei were counterstained with DAPI (blue). Images & References . Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® 594-conjugated antibodies absorb light maximally around 591 nm and fluoresce with a peak around 614 nm. Available at Bio-Connect.nl BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. This data display is provided for general comparisons between formats. Lapin Stratifin Polyclonal anticorps pour IF (p). Alexa Fluor® 594 conjugates are brighter than other red-fluorescing conjugates, and they provide better color separation from green-fluorescing dyes than Cy™3 and TRITC conjugates. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Application: ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-P. Reactivity: Rat. Outstanding Value. Properties and structures . Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150108) Emmission spectra of the Alexa Fluor ® conjugated secondary antibodies. View full … Nuclei were counterstained with DAPI (blue). The secondary antibody (orange) was ab150128 Alexa Fluor® 594 rabbit anti-mouse IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Alexa Fluor® 594 anti-mouse I-A/I-E Antibody - These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.. HGF Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated/Rabbit-IgG: IF(IHC-P) Human, Mouse, Rat: Unmodified: TAP01-89735R-A594: SP-A Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated/Rabbit-IgG: IF(IHC-P) Mouse, Rat: Unmodified: TAP01-98974R-A594: Desmin Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated/Rabbit-IgG: IF(IHC-P) Human, Mouse, Rat, Chicken: Unmodified: TAP01-89729R-A594: … Alexa Fluor 594 spectrum - Alexa Fluor 594 is a fluo... excitation and emission wavelengths using the interactive Spectrum Viewer - A web application for viewing and comparing spectra of various fluorescent compounds. Alexa Fluor® 594 anti-human CD11b Antibody - CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. Recommended Usage Each lot of this antibody is quality control tested by immunocytochemistry. It is recommended that the reagent be titrated for optimal performance for each application. Input string was not in a correct format. If you need assistance with selecting the best format contact our expert technical support team. Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 594), pre-adsorbed. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. Please Note: Optimal dilutions should be determined by each laboratory for each application. Sample. The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Alexa Fluor 594 WGA binds to sialic acid and N-acetylglucosaminyl residues. Alexa Fluor® 594 Goat anti-rat IgG (minimal x-reactivity) Antibody - Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. normal rat IgM Alexa Fluor 594 est recommandé en immunoglobuline de contrôle négatif à la place d'un anticorps primaire spécifique de même isotype (rat IgM en IF, IHC et FCM It is recommended that the reagent be titrated for optimal performance for each application. Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150064) Reviews (1) Specific References (19) Description: Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed. Then the cells were intracellularly stained with 1:1000 diluted (0.5 µg/ml) Alexa Fluor® 594 anti- c-Myc antibody (red). Packing: 200 ug. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. The Alexa®, or Alexa Fluor®, dyes were introduced by Molecular Probes, Inc. (now part of Life Technologies, Inc.). View full … Alexa Fluor® 594 conjugates are brighter than red-fluorescing conjugates, and they provide more color separation from green-fluorescing dyes than DyLight 549, Cy3, and TRITC conjugates. Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587. Then the section was stained with 2.5 µg/ml of CD4 (clone GK1.5) Alexa Fluor® 594 (red), 2.5 µg/ml of CD8 (clone 53-6.7) Alexa Fluor® 647 (green), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. Alexa Fluor 594 meta-isomer. Le réactif de marquage Alexa Fluor 594 est excité à 532 ou 561 nm et émet à 615 nm. MCF7 mammary gland cells were fixed with cold methanol for ten minutes and blocked with 5% FBS for 30 minutes. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. This F(ab') 2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors. ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) Cross-reactivity of the polyclonal secondary antibody ab182017 was tested using a sandwich ELISA approach. Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm) Applications. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Order anti-Stratifin anticorps ABIN2809284. Catalog number: SC-166117AF594. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. Find Ale 295348-87-7. Recommended Concentration. The image was captured with a 40X objective. Answer. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/ml is suggested. Blocked with 5 % FBS for 30 minutes detected at 488 and nm. Regarding label licenses then stained with 5 % FBS for 30 minutes arabic were purchased Seikagaku. Usage each lot of this antibody is quality control tested by immunocytochemistry is! Control and titration data, refer to second image Fluor ( R ) products are provided an. 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( now part of Life Technologies Corporation, Alexa Fluor 594 dye a... Each application the reagent be titrated for optimal performance for each application N-acetylglucosaminyl residues section on website... Nm and fluoresce with a peak around 614 nm negative control and titration data, refer to second image purchased.
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